Genome Biology

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Organization of the pronephric kidney revealed by large-scale gene expression mapping

Daniela Raciti1, Luca Reggiani1, Lars Geffers2, Qiuhong Jiang2, Francesca Bacchion1, Astrid E Subrizi1, Dave Clements3, Christopher Tindal3, Duncan R Davidson3, Brigitte Kaissling4 and André W Brändli1*

Author Affiliations

1 Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Wolfgang-Pauli-Strasse 10, CH-8093 Zürich, Switzerland

2 Department of Genes and Behavior, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany

3 MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK

4 Institute of Anatomy, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland

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Genome Biology 2008, 9:R84 doi:10.1186/gb-2008-9-5-r84

Published: 20 May 2008

Additional files

Additional data file 1:

Presented is a table listing marker gene expression in the developing Xenopus pronephric kidney at stages 25, 29/30, 35/36, and 40, as determined by whole-mount in situ hybridization. None of the genes analyzed were expressed in the stage 20 pronephric anlage. The expression levels were graded as follows: possible (+/-), present (+), and strong (++). The GenBank accession numbers of the cDNAs used for in situ hybridization probe synthesis are provided.

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Additional data file 2:

Presented is a table containing the annotation of marker gene expression in the Xenopus stage 35/36 pronephric kidney. The expression levels in the pronephros are indicated as follows: absent (-), possible (+/-), present (+), and strong (++). Abbreviations: CT, connecting tubule; DT, distal tubule; IT, intermediate tubule; PT, proximal tubule.

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Additional data file 3:

Presented is a table listing marker genes expressed in the proximal tubule of the stage 35/36 pronephric kidney, as determined by whole-mount in situ hybridization. Genes expressed exclusively in this compartment are indicated with asterisks.

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Additional data file 4:

Presented is a table listing marker genes expressed in the intermediate tubule of the stage 35/36 pronephric kidney, as determined by whole-mount in situ hybridization. Genes expressed exclusively in this compartment are indicated with asterisks.

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Additional data file 5:

Presented is a table listing marker genes expressed in the distal tubule of the stage 35/36 pronephric kidney, as determined by whole-mount in situ hybridization. Genes expressed exclusively in this compartment are indicated with asterisks.

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Additional data file 6:

Presented is a table listing marker genes expressed in the connecting tubule of the stage 35/36 pronephric kidney, as determined by whole-mount in situ hybridization. Genes expressed exclusively in this compartment are indicated with asterisks.

Format: PDF Size: 39KB Download file

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Open Data