Genome Biology

official impact factor 6.89

Open Access Research

Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus Magnaporthe oryzae

Yeonyee Oh1, Nicole Donofrio1,3, Huaqin Pan1,4, Sean Coughlan2, Douglas E Brown1, Shaowu Meng1, Thomas Mitchell1,5 and Ralph A Dean1*

Author Affiliations

1 North Carolina State University, Center for Integrated Fungal Research, Raleigh, NC 27695-7251, USA

2 Agilent Technologies, Little Falls, DE 19808-1644, USA

3 Current address: University of Delaware, Department of Plant and Soil Science, Newark, DE 19716, USA

4 Current address: RTI international, Research Triangle Park, NC 27709-2194, USA

5 Current address: Ohio State University, Department of Plant Pathology, Columbus, OH 43210, USA

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Genome Biology 2008, 9:R85 doi:10.1186/gb-2008-9-5-r85

Published: 20 May 2008

Additional files

Additional data file 1:

GO categorization (biological process) of differentially expressed genes during spore germination.

Format: XLS Size: 306KB Download file

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Additional data file 2:

Primer sequences for RT-PCR and qRT-PCR.

Format: XLS Size: 19KB Download file

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Additional data file 3:

Categorization of appressorium consensus genes with known function.

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Additional data file 4:

Differential expression of the putative melanin biosynthesis gene cluster during appressorium formation.

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Additional data file 5:

Adaptamer mediated PCR strategy for targeted gene deletion.

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Additional data file 6:

Primer sequences for gene specific replacement cassette construction.

Format: XLS Size: 33KB Download file

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Additional data file 7:

Confirmation of target gene replacement by PCR.

Format: DOC Size: 223KB Download file

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