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Gene expression response in target organ and whole blood varies as a function of target organ injury phenotype

Edward K Lobenhofer1*, J Todd Auman112, Pamela E Blackshear3, Gary A Boorman4, Pierre R Bushel5, Michael L Cunningham4, Jennifer M Fostel67, Kevin Gerrish2, Alexandra N Heinloth2, Richard D Irwin4, David E Malarkey8, B Alex Merrick7, Stella O Sieber2, Charles J Tucker2, Sandra M Ward8, Ralph E Wilson8, Patrick Hurban1, Raymond W Tennant9 and Richard S Paules10*

Author affiliations

1 Cogenics, a Division of Clinical Data, Inc., Morrisville, NC 27560, USA

2 NIEHS Microarray Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

3 Integrated Laboratory Systems, Inc., Research Triangle Park, NC 27709, USA

4 National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

5 Biostatistics Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

6 National Center for Toxicogenomics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

7 Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

8 Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

9 Cancer Biology Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

10 Environmental Stress and Cancer Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709

11 Current address: Institute for Pharmacogenomics and Individualized Therapy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA

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Citation and License

Genome Biology 2008, 9:R100  doi:10.1186/gb-2008-9-6-r100

Published: 20 June 2008

Abstract

This report details the standardized experimental design and the different data streams that were collected (histopathology, clinical chemistry, hematology and gene expression from the target tissue (liver) and a bio-available tissue (blood)) after treatment with eight known hepatotoxicants (at multiple time points and doses with multiple biological replicates). The results of the study demonstrate the classification of histopathological differences, likely reflecting differences in mechanisms of cell-specific toxicity, using either liver tissue or blood transcriptomic data.