Genetic analysis of the human infective trypanosome Trypanosoma brucei gambiense: chromosomal segregation, crossing over, and the construction of a genetic map

Anneli Cooper¹^,2 , Andy Tait¹ , Lindsay Sweeney¹ , Alison Tweedie¹ , Liam Morrison¹ , C Michael R Turner¹^,2 and Annette MacLeod¹

¹Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University Place, Glasgow, G12 8TA, UK

²Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University Place, Glasgow, G12 8TA, UK

author email corresponding author email

Genome Biology 2008, 9:R103doi:10.1186/gb-2008-9-6-r103


Published:	22 June 2008

Subject areas: Genetics, Genome studies, Medicine, Microbiology and parasitology

Additional files

Additional data file 1:

The segregation data for all the markers on each of the 11 Mb chromosomes is given. For each linkage group, markers are shown in map order, alongside: primer pair sequences, chromosomal location of the primers based on the available 927 sequence, estimated size of the PCR product, genotype of the STIB 386 parental line (AB), and inheritance pattern (either A or B) in the progeny clones for each marker. Novel sized alleles are marked as mutants.

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Additional data file 2:

The genetic maps of T. b. brucei isolate TREU 927 and T. b. gambiense isolate STIB 386 are shown alongside the TREU 927 physical map of every chromosome. The average physical size of a recombination unit between each marker is shown on the outside of each map in kb/cM and the genetic distance, given in cM, shown on the inside. Dashed lines link the position of all markers on the physical map to their relative position on the genetic maps, based on the TREU 927 sequence. Hot and cold spots are defined here as threefold more or less recombination than average for each genetic map and indicated against the physical map by red and blue bars, respectively.

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Additional data file 3:

A breakdown of the number of recombination events for every chromosome linkage group of every individual is given and the total and average for each individual and linkage group calculated.

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Additional data file 4:

The name and relevant genotypes of the parental strains and 38 unique F1 progeny derived from the STIB 386 × STIB 247 crosses that were analysed for the construction of the T. b. gambiense linkage map. Inheritance of marker alleles from both parents for 2 microsatellites (JS2 and PLC) and 3 minisatellites (CRAM, 292 and MS42) were used as genotyping markers.

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