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Summary of pancreas SAGE libraries generated |
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| Accession |
Stage |
Tissue subtype |
Cell types represented |
Library type |
Tags sequenced* |
Tag types |
|
|
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| SM161/SM244 |
TS17 |
Pdx1 EGFP+† |
All pancreas epithelial cells with the exception of rare Glucagon-positive cells |
Long SAGElite |
306,588 |
44,491 |
| SM231 |
TS19 |
Pdx1 EGFP+ |
All pancreas epithelial cells with the exception of rare Glucagon-positive cells |
Long SAGElite |
317,716 |
49,572 |
| SM162/SM245 |
TS20 |
Ngn3 EGFP-† |
A mixture of pancreas cell types composed predominantly of mesenchymal cells and pancreas epithelial progenitors as well as those destined to become exocrine cell types |
Long SAGElite |
308,745 |
47,695 |
| SM243/SM160 |
TS20 |
Ngn3 EGFP+ |
All endocrine progenitor cells as well as endocrine cells at various stages of maturation |
Long SAGElite |
320,473 |
51,847 |
| SM225/SM249 |
TS21 |
Ngn3 EGFP+ |
All endocrine progenitor cells as well as endocrine cells at various stages of maturation |
Long SAGElite |
313,503 |
58,864 |
| SM232 |
TS22 |
Ngn3 EGFP+ |
All endocrine progenitor cells as well as endocrine cells at various stages of maturation |
Long SAGElite |
301,222 |
37,726 |
| SM223 |
TS22 |
Whole |
A mixture of pancreas cell types composed predominantly of pancreas epithelial cells differentiating into exocrine cell types with some endocrine cells and mesenchymal cells |
Long SAGE |
98,189 |
13,676 |
| SM016 |
TS26 |
Whole |
A mixture of pancreas cell types composed predominantly of pancreas epithelial cells differentiating into exocrine cell types with some endocrine cells and mesenchymal cells |
Long SAGE |
81,130 |
17,963 |
| SM102 |
DPN70 |
Isolated ducts |
Hand picked adult ducts isolated by collagenase treatment and gradient centrifugation |
Long SAGE |
119,024 |
23,528 |
| SM017 |
DPN70 |
Isolated islets |
Hand picked adult islets isolated by collagenase treatment and gradient centrifugation composed of each of the major endocrine cell types |
Long SAGE |
99,968 |
16,039 |
|
*After 95% quality cutoffs for all tags. †The Pdx1 EGFP and Ngn3 EGFP transgenic strains were obtained from Douglas Melton as described in Gu et al. [22]. DPN, days post natal. | ||||||
Hoffman et al. Genome Biology 2008 9:R99 doi:10.1186/gb-2008-9-6-r99 |
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