Table 1

Summary of pancreas SAGE libraries generated

Accession
Stage
Tissue subtype
Cell types represented
Library type
Tags sequenced*
Tag types

SM161/SM244
TS17
Pdx1 EGFP+
All pancreas epithelial cells with the exception of rare Glucagon-positive cells
Long SAGElite
306,588
44,491
SM231
TS19
Pdx1 EGFP+
All pancreas epithelial cells with the exception of rare Glucagon-positive cells
Long SAGElite
317,716
49,572
SM162/SM245
TS20
Ngn3 EGFP-
A mixture of pancreas cell types composed predominantly of mesenchymal cells and pancreas epithelial progenitors as well as those destined to become exocrine cell types
Long SAGElite
308,745
47,695
SM243/SM160
TS20
Ngn3 EGFP+
All endocrine progenitor cells as well as endocrine cells at various stages of maturation
Long SAGElite
320,473
51,847
SM225/SM249
TS21
Ngn3 EGFP+
All endocrine progenitor cells as well as endocrine cells at various stages of maturation
Long SAGElite
313,503
58,864
SM232
TS22
Ngn3 EGFP+
All endocrine progenitor cells as well as endocrine cells at various stages of maturation
Long SAGElite
301,222
37,726
SM223
TS22
Whole
A mixture of pancreas cell types composed predominantly of pancreas epithelial cells differentiating into exocrine cell types with some endocrine cells and mesenchymal cells
Long SAGE
98,189
13,676
SM016
TS26
Whole
A mixture of pancreas cell types composed predominantly of pancreas epithelial cells differentiating into exocrine cell types with some endocrine cells and mesenchymal cells
Long SAGE
81,130
17,963
SM102
DPN70
Isolated ducts
Hand picked adult ducts isolated by collagenase treatment and gradient centrifugation
Long SAGE
119,024
23,528
SM017
DPN70
Isolated islets
Hand picked adult islets isolated by collagenase treatment and gradient centrifugation composed of each of the major endocrine cell types
Long SAGE
99,968
16,039

*After 95% quality cutoffs for all tags. The Pdx1 EGFP and Ngn3 EGFP transgenic strains were obtained from Douglas Melton as described in Gu et al. [22]. DPN, days post natal.

Hoffman et al. Genome Biology 2008 9:R99   doi:10.1186/gb-2008-9-6-r99