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Susceptibility to glaucoma: differential comparison of the astrocyte transcriptome from glaucomatous African American and Caucasian American donors

Thomas J Lukas1*, Haixi Miao2, Lin Chen2, Sean M Riordan2, Wenjun Li2, Andrea M Crabb2, Alexandria Wise3, Pan Du4, Simon M Lin4 and M Rosario Hernandez2

Author Affiliations

1 Department of Molecular Pharmacology and Biological Chemistry, Feinberg School of Medicine, Northwestern University, E Chicago Ave, Chicago, IL 60611 USA

2 Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, E Chicago Ave, Chicago, IL 60611 USA

3 Department of Biology, City College of New York, Convent Ave, New York, NY 10031, USA

4 Robert H Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, E Chicago Ave, Chicago, IL 60611 USA

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Genome Biology 2008, 9:R111  doi:10.1186/gb-2008-9-7-r111

Published: 9 July 2008

Additional files

Additional data file 1:

Clinical information about CAG and AAG eyes used to generate primary cultures of ONH astrocytes.

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Additional data file 2:

Demographic information of CA and AA normal donor eyes used to generate primary cultures of ONH astrocytes.

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Additional data file 3:

Probe-sets on the chip and used in analysis.

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Additional data file 4:

Primers used for qRT-PCR.

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Additional data file 5:

Includes the comparison between AAG versus normal AA.

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Additional data file 6:

Differentially expressed genes between CAG and normal CA.

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Additional data file 7:

Differentially expressed genes between AAG and CAG.

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Additional data file 8:

Differentially expressed genes in ONH astrocytes from AAG compared to both normal AA and CAG.

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Additional data file 9:

GO comparisons for AAG versus CAG expression sets.

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Additional data file 10:

Common genes were selected from the GO lists (Additional data files 7-9) for each dataset (AAG-CAG, AAG-AA, and CAG-CA comparisons). The fraction of common genes (y-axis) for the GO terms 'phosphorylation' (grey bar) and 'cell-cell signaling' categories (black bar) are shown.

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Additional data file 11:

(a-e) CAG-CA and comparison: CALM (a), CAPG (b), GJA1 (c), GPNMB (d) and SOD2 (e). (f-j) AAG-AA comparison: GSTA4 (f), LOXL2 (g), MYH10 (h), PDLIM7 (i) and OLR1 (j). Genes were normalized to 18S. Graphical representation of the relative mRNA levels in normal and glaucomatous AA and normal and glaucomatous CA astrocytes (n = 6, two-tailed t-test was used. Asterisk indicates p < 0.05).

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