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Open Access Research

The transcriptional program underlying the physiology of clostridial sporulation

Shawn W Jones123, Carlos J Paredes14, Bryan Tracy1, Nathan Cheng15, Ryan Sillers1, Ryan S Senger23 and Eleftherios T Papoutsakis23*

Author Affiliations

1 Department of Chemical and Biological Engineering, Northwestern University, Sheridan Road, Evanston, IL 60208-3120, USA

2 Department of Chemical Engineering, University of Delaware, Academy Street, Newark, DE 19716, USA

3 Delaware Biotechnology Institute, University of Delaware, Innovation Way, Newark, DE 19711, USA

4 Current address: Cobalt Biofuels, Clyde Avenue, Mountain View, CA 94043, USA

5 Current address: The Zitter Group, New Montgomery Street, San Francisco, CA 94105, USA

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Genome Biology 2008, 9:R114  doi:10.1186/gb-2008-9-7-r114

Published: 16 July 2008

Additional files

Additional data file 1:

Comparison of the present microarray study to an earlier microarray study that examined the early sporulation of C. acetobutylicum.

Format: PDF Size: 19KB Download file

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Open Data

Additional data file 2:

COG analysis for each cluster and all the genes placed in each cluster.

Format: XLS Size: 117KB Download file

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Open Data

Additional data file 3:

Transcriptional profiles, in terms of both intensity and differential expression, of specific gene clusters.

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Open Data

Additional data file 4:

Includes a brief discussion on how the genes used to construct the deduced activity plots were chosen.

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Open Data

Additional data file 5:

Differential expression and intensity of all annotated histidine kinases and response regulators.

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Open Data

Additional data file 6:

Phylogenetic tree resulting from the alignment of the σ70-related and unannotated sigma factors from ten bacterial species.

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Additional data file 7:

Sequences for each asRNA construct.

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Open Data

Additional data file 8:

TEM images of the plasmid control strain, asCAP0167, and asCAC1766.

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Open Data

Additional data file 9:

Primer sequences used in the Q-RT-PCR experiments.

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Open Data