Open Access Research

Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy

Jean-Michel Ubeda1, Danielle Légaré1, Frédéric Raymond12, Amin Ahmed Ouameur1, Sébastien Boisvert2, Philippe Rigault2, Jacques Corbeil12, Michel J Tremblay1, Martin Olivier3, Barbara Papadopoulou1 and Marc Ouellette1*

Author Affiliations

1 Université Laval, Division de Microbiologie, Centre de Recherche en Infectiologie, boulevard Laurier, Québec, G1V 4G2, Canada

2 Université Laval, Centre de Recherche en Endocrinologie Moléculaire et Oncologique, boulevard Laurier, Québec, G1V 4G2, Canada

3 McGill University, Department of Microbiology and Immunology, Lyman Duff Medical Building, University Street, Montreal, H3A 2B4, Canada

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Genome Biology 2008, 9:R115  doi:10.1186/gb-2008-9-7-r115

Published: 18 July 2008

Additional files

Additional data file 1:

Differential expression measured by the full-genome microarray analysis.

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Additional data file 2:

Figure S1 shows the direct repeats flanking the DHFR-TS locus of L. major and L. infantum chromosome 6, and also provides the circular junction sequence formed by homologous recombination. Figure S2 shows the inverted repeats present on chromosome 23 of L. infantum, and provides the sequence of the new junction formed through the inverted duplication. Figure S3 shows the sequence of the L. major chimera gene LmjF10.0380/0390.

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Additional data file 3:

Results of the comparative genomic hybridization analyses of L. major MTX60.4 versus the respective wild-type cells.

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