Figure 9.

Peptide evidence indicating alternative frame shift. The position of ORF XII-4-5562689-5562144 in the genome scaffold is indicated by a red line on the grey track at the top of the figure and this region is expanded below, the red triangle demarking the ORF length. Predicted exons are indicated as red shaded boxes, linked by zigzag lines to indicate the position of exon/intron boundaries. Peptides aligning with this region are shown in yellow. The gene of interest with the release4 annotation (50.m05694) is highlighted in blue. Predicted sequences for this gene and the ORF and TgGlmHMM_1717 are shown as inserts. Sequence for which there is matching peptide evidence is shown in red. TgGlmHMM_1717 comprises several exons and the complete sequence is not given; the start methionine is shown in green. Mass spectrometric evidence for one peptide sequence derived by the 1-DE approach is shown.