Molecular evolutionary analyses of candidate GPCRs shown to physically interact with GPA1. The Arabidopsis, Oryza, and Populus proteomes were subjected to BLAST analyses (e-20 cutoff) using our positive interacting candidate GPCR protein sequences (filled triangles). Multiple sequence alignments were created in ClustalX and evolutionary relationships were estimated using the neighbor joining method with 1,000 bootstrap replicates. Sequences identified by our bioinformatic pipeline as candidate GPCRs are indicated with empty triangles, with upward pointing triangles indicating those found within our high ranking candidate sets and downward pointing triangles indicating those present in the second tier. Scale bars indicate evolutionary distance as measured by residue substitutions per site. (a) RGS1; (b) GCR1; (c) Cand2 and Cand8; (d) Cand1; (e) Cand3, 4, and 5; (f) Cand6 and Cand7; and (g) HHP2.
Gookin et al. Genome Biology 2008 9:R120 doi:10.1186/gb-2008-9-7-r120