Table 3

Results for the split-ubiquitin system protein-protein interaction assays between GPA1 Nub fusion proteins and the candidate GPCR-Cub-PLV fusion proteins

GPA1 Nub fusion orientations

Locus tested (X-Cub-PLV)

Name

Nub_wt-GPA1

Nub_G-GPA1

GPA1-Nub_wt

GPA1-Nub_G

At1g57680.1

Cand1

++

-

++

-

At3g05010.1

Cand2

++

-

+

-

At3g59090.1

Cand3

++

-

++

-

At3g59090.3

Cand5

++

-

++

-

At5g18520.1

Cand7

++

-

++

-

At5g27210.1

Cand8

++

-

-

-

At4g21790.1

TOM1

++

-

++

-

At4g30850.1

HHP2

++

-

+

-

Results were recorded after 3-5 days of yeast growth on minimal media containing 1 mM methionine to identify proteins that interact specifically with GPA1 as indicated by the extent of yeast growth. (+) indicates moderate growth while (++) indicate heavy yeast growth. Empty vector control plates did not show growth (data not shown). X-Cub-PLV, candidate GPCR-Cub-PLV fusions. Nub_wt, wild type amino-terminal half of ubiquitin. NubG, reduced affinity mutant of Nub_wt.

Gookin et al. Genome Biology 2008 9:R120   doi:10.1186/gb-2008-9-7-r120