The identification of differential exon splicing by RNAseq. In this hypothetical genome-browser view, RNAseq tags (shown in red) are aligned to the genome sequence, giving a quantitative view of tag densities across the locus. Genome-aligned reads identify individual exons and exon-exon junction usage can be monitored by matching tags to a reference set of junction sequences. Differential exon-exon junction usage can be used to identify canonical and alternative splicing events.
Cloonan and Grimmond Genome Biology 2008 9:234 doi:10.1186/gb-2008-9-9-234