Theoretical and observed mBAF values for different types of allelic imbalances with increasing tumor content for the CRL-2324 dilution series. (a) Linearity of segmented mBAF values for the copy neutral LOH region on 13q21.31-qter across the CRL-2324 dilution series. The red line corresponds to expected mBAF values and the gray line to a linear regression fit for experimental data between 0% and 100% tumor content, for which c represents the tumor fraction. (b) Theoretical mBAF values for different allelic imbalances: hemizygous loss (black line), copy number neutral LOH (red line), single copy gain (green line), and 4N amplification, for example, BBBA (blue line).
Staaf et al. Genome Biology 2008 9:R136 doi:10.1186/gb-2008-9-9-r136