Figure 4.

Overrepresented sequence motifs present at the 5' end of intronic regions associated with splice junctions regulated by the (a) wingless and (b) insulin pathways. Motifs were derived from a dataset of sequences corresponding to the 50 nucleotides of introns flanking splice junctions that change upon activation of a signaling pathway, as well as the corresponding regions in the same intron of the other 11 Drosophila species. Motifs were identified using MEME and PHYLOGIBBS software and the specificity of the enrichment assessed with a set of control sequences derived from constitutive and alternative splice junctions that do not change upon activation of the signaling pathway. A detailed account of motifs and statistical assessment of their significance can be found in [63]. Represented are the relative frequencies of each nucleotide at each position in the nine nucleotide motifs. Genes containing the junctions included in each of the motifs are as follow. Insulin motif (44): sbb, cg15611, graf, cg7995, cg13213, cul-2, cher, ald, cg6265, cg7950, cg1021, cg7059, tomosyn, cg8036, cg1141, wdb, cg3168, cg8789, cg32425, cg16833, cg13499, cg4502, cg31732, cg32103, cg33085, sesB, scb, sdc, nemy, Ef2b, keap1, drpr, cg15105, : cg5059, spi, cg6231, cg14869, cpx, spri, cg16758, dom, Ca-P60A, ptp99A, cg33130. Wingless motif (10): stat92E, trx, cg2747, smi35A, hph, ced-6, cg33130, slo, cg4502, cg5794.