Strand-specific RT-PCR. Lanes 1 to 6 represent the amplification of the Sox4 and Sox11 transcripts using more than one probe. Lane 7 shows the amplification of the Hmbs housekeeping gene whereas lane 8 represents the amplification of water as non-template control (NTC) using the primer set for Hmbs. The first panel consists of amplicons generated from the reverse-transcribed sense strand cDNA whereas the second panel consists of amplicons generated from the reverse-transcribed antisense strand cDNA. The third panel represents amplicons generated from oligo-d [T]15 primed reverse-transcribed cDNA, which serves as a positive control. The last panel represents amplicons generated from primer-free reverse-transcription reactions. The numbers on the left indicate the size of the generated bands.
Ling et al. Genome Biology 2009 10:R104 doi:10.1186/gb-2009-10-10-r104