Table 4

RT-qPCR validation of SAGE profile for P1.5 versus adult stages

Fold change


SAGE tag

RefSeq accession

Gene ID

Adult/P1.5 (SAGE)

Adult/P1.5 (RT-qPCR)


aaattattgggaaatcc

NM_011123

proteolipid protein (myelin) 1, Plp1

38.9

43.33

tttcagcagtgttggct

NM_013556

hypoxanthine guanine phosphoribosyl transferase 1, Hprt1

6.99

2.26

actcggagccaccagac

NM_009790

calmodulin 1, Calm1

4.57

1.83

gcttcgtccacacagcg

NM_010777

myelin basic protein, Mbp

As

1.79

tccccgtcat

NM_026106

down-regulator of transcription 1, Dr1

Ps

-1.46

gggaaactaagggagag

NM_172503

zinc finger, SWIM domain containing 4, Zswim4

-42.42

-1.96*

gaacgcaagttcagccc

NM_031404

actin-like 6B, Actl6b

-5.51

-2.26

gtgaaactaaaaaaaaa

NM_009094

ribosomal protein S4, X-linked, Rps4x

-4.72

-2.27

Nil

NM_009726

ATPase, Cu++ transporting, alpha polypeptide, Atp7a

Nil

-2.91

agaagtgtttggagttt

NM_008253

high mobility group box 3, Hmgb3

-20.99

-3.53

cctttgtgacagtggcc

NM_025635

ZW10 interactor, Zwint

4.65

-4.04

acagtctatgttggagg

BQ177886/NM_010487

C57BL/6 whole brain E15.5 (or known as embryonic lethal, abnormal vision, Drosophila-like 3 (Hu antigen C), Elavl3)

-41.99

-4.06

gatacttggaatgacta

NM_007393

actin, beta, cytoplasmic, Actb

-18.49

-4.35

ctggcttctt

NM_008538

myristoylated alanine rich protein kinase C substrate, Marcks

Ps

-4.76

Nil

NM_025958

cullin-associated and neddylation-dissociated 2 (putative), Cand2

Nil

-5.62

gctttgactgttctctt

NM_009870

cyclin-dependent kinase 4, Cdk4

-56.55

-5.77

ctcagtaatg

AA122503

EST from M2 cells of skin melanoma

-12.28

-11.12

tggagcgttggctgtat

NM_009238

SRY-box containing gene 4, Sox4

-31.51

-11.74

cacgacaccccccaccc

NM_007792

cysteine and glycine-rich protein 2, Csrp2

Ps

-26.10

tgtgtgaggtgttgtga

NM_009559

zinc finger protein 57, Zfp57

-16.33

-32.74

gggacctcgtggaagcc

NM_010025

doublecortin, Dcx

-24.56

-82.11


All RT-qPCR data are statistically significant at P < 0.001 unless specified: * P < 0.01. A disagreement between RT-qPCR and SAGE data. As: adult-specific expression; Nil: SAGE data not available; Ps: P1.5-specific expression. Fold change values of <1.0 are presented in a negative fold change format.

Ling et al. Genome Biology 2009 10:R104   doi:10.1186/gb-2009-10-10-r104

Open Data