Table 4 |
||||
|
RT-qPCR validation of SAGE profile for P1.5 versus adult stages |
||||
|
Fold change |
||||
|
|
||||
|
SAGE tag |
RefSeq accession |
Gene ID |
Adult/P1.5 (SAGE) |
Adult/P1.5 (RT-qPCR) |
|
|
||||
|
aaattattgggaaatcc |
proteolipid protein (myelin) 1, Plp1 |
38.9 |
43.33 |
|
|
tttcagcagtgttggct |
hypoxanthine guanine phosphoribosyl transferase 1, Hprt1 |
6.99 |
2.26 |
|
|
actcggagccaccagac |
calmodulin 1, Calm1 |
4.57 |
1.83 |
|
|
gcttcgtccacacagcg |
myelin basic protein, Mbp |
As |
1.79 |
|
|
tccccgtcat |
down-regulator of transcription 1, Dr1 |
Ps |
-1.46 |
|
|
gggaaactaagggagag |
zinc finger, SWIM domain containing 4, Zswim4 |
-42.42 |
-1.96* |
|
|
gaacgcaagttcagccc |
actin-like 6B, Actl6b |
-5.51 |
-2.26 |
|
|
gtgaaactaaaaaaaaa |
ribosomal protein S4, X-linked, Rps4x |
-4.72 |
-2.27 |
|
|
Nil |
ATPase, Cu++ transporting, alpha polypeptide, Atp7a |
Nil |
-2.91 |
|
|
agaagtgtttggagttt |
high mobility group box 3, Hmgb3 |
-20.99 |
-3.53 |
|
|
cctttgtgacagtggcc |
ZW10 interactor, Zwint |
4.65 |
-4.04† |
|
|
acagtctatgttggagg |
C57BL/6 whole brain E15.5 (or known as embryonic lethal, abnormal vision, Drosophila-like 3 (Hu antigen C), Elavl3) |
-41.99 |
-4.06 |
|
|
gatacttggaatgacta |
actin, beta, cytoplasmic, Actb |
-18.49 |
-4.35 |
|
|
ctggcttctt |
myristoylated alanine rich protein kinase C substrate, Marcks |
Ps |
-4.76 |
|
|
Nil |
cullin-associated and neddylation-dissociated 2 (putative), Cand2 |
Nil |
-5.62 |
|
|
gctttgactgttctctt |
cyclin-dependent kinase 4, Cdk4 |
-56.55 |
-5.77 |
|
|
ctcagtaatg |
EST from M2 cells of skin melanoma |
-12.28 |
-11.12 |
|
|
tggagcgttggctgtat |
SRY-box containing gene 4, Sox4 |
-31.51 |
-11.74 |
|
|
cacgacaccccccaccc |
cysteine and glycine-rich protein 2, Csrp2 |
Ps |
-26.10 |
|
|
tgtgtgaggtgttgtga |
zinc finger protein 57, Zfp57 |
-16.33 |
-32.74 |
|
|
gggacctcgtggaagcc |
doublecortin, Dcx |
-24.56 |
-82.11 |
|
|
|
||||
|
All RT-qPCR data are statistically significant at P < 0.001 unless specified: * P < 0.01. †A disagreement between RT-qPCR and SAGE data. As: adult-specific expression; Nil: SAGE data not available; Ps: P1.5-specific expression. Fold change values of <1.0 are presented in a negative fold change format. |
||||
|
Ling et al. Genome Biology 2009 10:R104 doi:10.1186/gb-2009-10-10-r104 |
||||
