Table 6

RT-qPCR validation of SAGE profile for rostral E15.5 and caudal E15.5 regions

Fold change


SAGE tag

RefSeq accession

Gene ID

Caudal/rostral (SAGE)

Caudal/rostral (RT-qPCR)


gttgttcttccagtcgg

NM_016916

bladder cancer associated protein homolog (human), Blcap

2.68

1.43

gtcatagctgttctgtg

BC025816

EST sequence BC025816

Cs

1.31 (NS)

aagcttgacatttggaa

NM_026187

ankyrin repeat and zinc finger domain containing 1, Ankzf1

Rs

1.17(NS)

gatacttggaatgacta

NM_007393

actin, beta, cytoplasmic, Actb

-2.63

-1.32

ttggtgaaggaaaaaac

NM_021278

thymosin, beta 4, X chromosome, Tmsb4x

-2.33

-2.13 (NS)


All RT-qPCR data are statistically significant at P < 0.05 unless specified. A disagreement between RT-qPCR and SAGE data. Cs: caudal region-specific expression; NS: no statistically significant difference between two developmental stages; Rs: rostral region-specific expression. Fold change values of <1.0 are presented in a negative fold change format.

Ling et al. Genome Biology 2009 10:R104   doi:10.1186/gb-2009-10-10-r104

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