Table 6 |
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|
RT-qPCR validation of SAGE profile for rostral E15.5 and caudal E15.5 regions |
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|
Fold change |
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|
|
||||
|
SAGE tag |
RefSeq accession |
Gene ID |
Caudal/rostral (SAGE) |
Caudal/rostral (RT-qPCR) |
|
|
||||
|
gttgttcttccagtcgg |
bladder cancer associated protein homolog (human), Blcap |
2.68 |
1.43 |
|
|
gtcatagctgttctgtg |
EST sequence BC025816 |
Cs |
1.31 (NS) |
|
|
aagcttgacatttggaa |
ankyrin repeat and zinc finger domain containing 1, Ankzf1 |
Rs |
1.17† (NS) |
|
|
gatacttggaatgacta |
actin, beta, cytoplasmic, Actb |
-2.63 |
-1.32 |
|
|
ttggtgaaggaaaaaac |
thymosin, beta 4, X chromosome, Tmsb4x |
-2.33 |
-2.13 (NS) |
|
|
|
||||
|
All RT-qPCR data are statistically significant at P < 0.05 unless specified. †A disagreement between RT-qPCR and SAGE data. Cs: caudal region-specific expression; NS: no statistically significant difference between two developmental stages; Rs: rostral region-specific expression. Fold change values of <1.0 are presented in a negative fold change format. |
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|
Ling et al. Genome Biology 2009 10:R104 doi:10.1186/gb-2009-10-10-r104 |
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