Method
Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels
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* Corresponding author: David J Adams da1@sanger.ac.uk
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1 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1HH, UK
2 Mammalian Genetics Unit, MRC-Harwell, Harwell Science and Innovation Campus, Oxfordshire, OX11 ORD, UK
3 Department of Genetics, Case Western Reserve University, Adelbert Rd, Cleveland, OH 44106-4955. USA
Genome Biology 2009, 10:R112 doi:10.1186/gb-2009-10-10-r112
Published: 13 October 2009Abstract
Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived strain A/J, and the Mus musculus castaneus derived strain CAST/Ei. We describe approaches for the accurate identification of nucleotide and structural variation in the genomes of vertebrate experimental organisms, and show how these techniques can be applied to help prioritize candidate genes within quantitative trait loci.