Table 5 |
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|
Assembly statistics |
|||||||||
|
Assembler |
Sequencing |
Strains |
Insert size |
Read length (bp) |
Number of reads |
Raw read coverage |
Assembled bases (Mb) |
Contig coverage |
Contig N50 |
|
|
|||||||||
|
FuzzyPath |
Illumina |
CAST/Ei |
200 bp |
36 |
173 million |
65× |
76.03 |
80.0% |
2,315 |
|
Velvet* |
Illumina |
CAST/Ei |
200 bp |
36 |
173 million |
65× |
58.09 |
61.15% |
391 |
|
ABySS |
Illumina |
CAST/Ei |
200 bp |
36 |
173 million |
65× |
72.80 |
76.63% |
1,022 |
|
FuzzyPath |
Illumina |
A/J |
200 bp |
36 |
112 million |
42× |
55.87 |
58.8% |
959 |
|
Phusion |
Capillary |
A/J |
3-5 kb |
400-900 |
357,100 |
2.5× |
81.2 |
85.5% |
3,377 |
|
FuzzyPath |
Hybrid |
A/J† |
Hybrid† |
Hybrid† |
Hybrid† |
Hybrid† |
85.54 |
90.0% |
5,793 |
|
|
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|
*Version 0.4. We also tried the latest version 0.7, which had been much improved in terms of contig length, but ran out of memory on a computer with 192 Gb RAM. †Assembly incorporating the A/J Illumina reads and Celera capillary reads. |
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|
Sudbery et al. Genome Biology 2009 10:R112 doi:10.1186/gb-2009-10-10-r112 |
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