Table 5

Assembly statistics

Assembler

Sequencing

Strains

Insert size

Read length (bp)

Number of reads

Raw read coverage

Assembled bases (Mb)

Contig coverage

Contig N50


FuzzyPath

Illumina

CAST/Ei

200 bp

36

173 million

65×

76.03

80.0%

2,315

Velvet*

Illumina

CAST/Ei

200 bp

36

173 million

65×

58.09

61.15%

391

ABySS

Illumina

CAST/Ei

200 bp

36

173 million

65×

72.80

76.63%

1,022

FuzzyPath

Illumina

A/J

200 bp

36

112 million

42×

55.87

58.8%

959

Phusion

Capillary

A/J

3-5 kb

400-900

357,100

2.5×

81.2

85.5%

3,377

FuzzyPath

Hybrid

A/J

Hybrid

Hybrid

Hybrid

Hybrid

85.54

90.0%

5,793


*Version 0.4. We also tried the latest version 0.7, which had been much improved in terms of contig length, but ran out of memory on a computer with 192 Gb RAM. Assembly incorporating the A/J Illumina reads and Celera capillary reads.

Sudbery et al. Genome Biology 2009 10:R112   doi:10.1186/gb-2009-10-10-r112

Open Data