Cell cycle changes during the day-night cycle of harvesting. Example DNA content histograms of nuclear extracts taken from 1N cultures at different times are shown. The time point at 15 h on day 1 is not shown but had a similar distribution to that at 19 h on day 1 and 15 h30 on day 2. RNA was not collected at 15 h30 on day 2, but nuclear extracts (shown here), flow cytometric profiles, and Fv/Fm confirmed cells had returned to the same state after a complete diel cycle. Extracted nuclei were stained with Sybr Green I and analyzed by flow cytometry.
von Dassow et al. Genome Biology 2009 10:R114 doi:10.1186/gb-2009-10-10-r114