Venn diagram showing the degree of overlap existing E. huxleyi EST libraries. Included are the libraries analyzed in this study (1N RCC1217 and 2N RCC1216, combined) and the two other publicly available EST libraries (CCMP 1516 and CCMP371). ESTs were considered matching based on BLAT criteria of an alignment length of ≥ 100 nucleotides and ≥ 95% identity. The degrees of overlap increased only very modestly when the BLAT criteria were relaxed to an alignment length of ≥ 50 nucleotides.
von Dassow et al. Genome Biology 2009 10:R114 doi:10.1186/gb-2009-10-10-r114