Sequences from NUP214-XKR3 fusion transcripts detected after hybrid selection. After hybrid selection, 152 reads were aligned to the transcriptome and detected as NUP214-XKR3 fusions. From top to bottom, we observed 137, four, eight, and three reads for these transcripts. The NUP214 (exon 27) to XKR3 (exon 4) has a stop codon downstream (not shown). Only NUP214 (exon 29) to XKR3 (exon 4) retains an open reading frame downstream of the fusion. Before hybrid selection, eight reads were aligned to the transcriptome and detected as NUP214-XKR3 fusions; only the NUP214 (exon 29) to XKR3 (exon 2) transcript was detected. Sequence from NUP214 DNA is shown as lower case, and from XKR3, as bold and upper case.
Levin et al. Genome Biology 2009 10:R115 doi:10.1186/gb-2009-10-10-r115