Table 1

Analysis of Illumina sequence in cDNA hybrid selection

Sequence filter criteria

Beforea

Aftera


Total purity-filtered reads

7,907,124

7,635,761

Aligned to all transcriptome

4,515,009

6,664,152

Unique in transcriptome

4,303,769

6,508,099

Mapping to 1 of 887 target transcripts

220,151

6,364,131

On-target specificity

5%

98%


aNumber of sequence reads before and after hybrid selection.

Levin et al. Genome Biology 2009 10:R115   doi:10.1186/gb-2009-10-10-r115

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