Research
Simultaneous transcription of duplicated var2csa gene copies in individual Plasmodium falciparum parasites
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* Corresponding author: Qijun Chen qijun.chen@smi.se
- † Equal contributors
1 Department of Microbiology, Tumor and Cell Biology, Nobels Väg 16, Karolinska Institutet, SE-171 77 Stockholm, Sweden
2 Swedish Institute for Infectious Disease Control, Nobels Väg 18, SE-171 82, Stockholm, Sweden
3 Department of Cell and Molecular Biology, Uppsala University, Husargatan 3, SE-751 21 Uppsala, Sweden
4 Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Xi An Da Lu 5333, Changchun 130062, China
5 Laboratory of Parasitology, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Dong Dan San Tiao 9, Beijing 100730, China
Genome Biology 2009, 10:R117 doi:10.1186/gb-2009-10-10-r117
Published: 22 October 2009Additional files
Additional data file 1:
Graphs showing standard curves of amplifications using primer pairs towards (a) var2csa DBL2X, (b) var2csa DBL4ε and (c) Pf332 S326P together with detection by allele-specific FAM- or VIC-labeled probes. Serial dilutions of HB3 gDNA were used in all reactions. Filled squares show amplification detected with FAM-labeled probe and filled circles detection with VIC-labeled probe. Amplification efficiencies for primers and probes within all respective allele assays were sufficiently close to obviate the need for a correction factor.
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