Table 1 |
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Primer and probe sequences |
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Target gene (application) |
Primers |
Probes |
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seryl-tRNA synthesase (real-time PCR) |
F: TATCATCTCAACAGGTATCTACATCTCCTA |
NA |
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R: TTTGAGAGTTACATGTGGTATCATCTTTT |
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β-tubulin (real-time PCR) |
F: CGTGCTGGCCCCTTTG |
NA |
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R: TCCTGCACCTGTTTGACCAA |
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var2csa DBL2x (real-time PCR) |
F: AATCATGGTGGAACACGAACAA |
6-FAM-ATATTTGGTTAGCAATGAA-MGB (allele 1) |
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R: AACTACTACCACTACCAGTGACACTACCAT |
6-VIC-ATATATTTGGACAGCAATGA-MGB (allele 2) |
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var2csa DBL4ε (real-time PCR) |
F: TATACCGTAAAAGTAACAAAGAATCGGAA |
6-FAM-ATGATTATGGAACCTACAGTC-MGB (allele 1) |
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R: ACAACTACAACAAATGTAGTTCCCATTAATT |
6-VIC-ATGATTATGGCACCTACAGT-MGB (allele 2) |
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Pf332 S326P (real-time PCR) |
F: AGTGGTTCCCAAGAAGAAGAACAA |
6-FAM-ATGATGTGTCACATAGGA-MGB (wild type) |
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R: ACATTCTGTCATCTAATTTATCCAGTAGAGAT |
6-VIC-ATGTGCCACATAGGA-MGB (Mutant) |
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var2csa nest 1 (single cell PCR) |
F: ACTTGAAAATGTGTGCAAAGGAGTA |
NA |
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R: TTACCCAGTGGAGACGGAACAT |
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var2csa lr1 (DNA-FISH/Southern blot) |
F: AAAATTACTGTGAATCATTCAGAT |
NA |
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R: AAGGTGTTTCAGACGAAGTATTAGCAT |
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var2csa lr2 (DNA-FISH) |
F: ACTGTGAATGTTACGAATTGTGGATAA |
NA |
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R: ACATTTCCCCCTCAATTCCTTT |
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var2csa PFHG_05046.1_Sp6_a (RNA-FISH, detecting mRNA) |
F: TCATTTAGGTGACACTATAGAAGTGTATATTTT GGGTCATCATTTCGATATTT |
NA |
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R: GAGATGAAAACAAGTGTGAAACAAAATC |
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var2csa PFHG_05046.1_Sp6_s (RNA-FISH, detecting antisense) |
F: TCATTTAGGTGACACTATAGAAGAGATGAAAA CAAGTGTGAAACAAAATCC |
NA |
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R: TGTATATTTTGGGTCATCATTTCGAT |
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var2csa PFHG_05155.1_Sp6_a (RNA-FISH, detecting mRNA) |
F: TCATTTAGGTGACACTATAGAAGTGCTTATAA TTTTCATCATCATTTGGATATTTATC |
NA |
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R: CAGATCAATTGAAAGGTTCTAACATTTT |
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var2csa PFHG_05155.1_Sp6_s (RNA-FISH, detecting antisense) |
F: TCATTTAGGTGACACTATAGAAGAGATCAATT GAAAGGTTCTAACATTTTAGAAC |
NA |
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R: CTTATAATTTTCATCATCATTTGGATATTTATC |
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kahrp_Sp6_a (RNA-FISH, detecting mRNA) |
F: TCATTTAGGTGACACTATAGAAGAGGTTGGTG AACCTGTGGTGCTT |
NA |
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R: AACTTTAGCACAAAAGCAACATGAA |
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kahrp_Sp6_s (RNA-FISH, detecting antisense) |
F: TCATTTAGGTGACACTATAGAAGAGAACTTTA GCACAAAAGCAACATGAA |
NA |
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R: GTTGGTGAACCTGTGGTGCTT |
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F, forward; NA, not applicable; R, reverse. |
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Brolin et al. Genome Biology 2009 10:R117 doi:10.1186/gb-2009-10-10-r117 |
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