Research
Whole-genome resequencing of Escherichia coli K-12 MG1655 undergoing short-term laboratory evolution in lactate minimal media reveals flexible selection of adaptive mutations
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* Corresponding author: Bernhard Ø Palsson bpalsson@bioeng.ucsd.edu
1 Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, California, 92093-0332, USA
2 Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, California, 92093-0412, USA
3 Department of Computer Science, Virginia Commonwealth University, 401 West Main Street, Richmond, Virginia, 23284-3019, USA
4 Center for the Study of Biological Complexity, Virginia Commonwealth University, 1000 W. Cary St., Richmond, Virginia, 23284-3068, USA
Genome Biology 2009, 10:R118 doi:10.1186/gb-2009-10-10-r118
Published: 22 October 2009Additional files
Additional data file 1:
Mutations reported for LactA, LactB, LactC, LactD, and LactE strains using Nimblegen CGS arrays.
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Additional data file 2:
Mutations reported for all strains using Solexa sequencing.
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Additional data file 3:
False negative rate of our mutation detection algorithm using a reference sequence genome with 'mutations' inserted at known locations.
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Additional data file 4:
Regulons enriched for differential expression in LactA, LactB, LactC, LactD, and LactE strains.
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Additional data file 5:
The presence and absence of mutations at time points or in colonies were used for determination of mutation trajectory and population mutation penetration.
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Additional data file 6:
Primers used in this study.
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