ChIP-validated network. The genes predicted to be regulated by any of 12 TFs (CBFB, IRF8, NFKB, NYFA, MXI1, PCGF4, RARA, RXRA, SP1, SPI1, RXRB and UHRF1) were examined for TF binding to their proximal promoter regions by X-ChIP/qPCR. Seventy out of 113 edges were validated as TF-binding positives, strong candidates for direct regulatory edges.
Tomaru et al. Genome Biology 2009 10:R121 doi:10.1186/gb-2009-10-11-r121