Additional data file 2.
Western blotting with specific antibodies against each of the TFs and the protein extracts prepared from the THP-1 cells transfected with 20 nM negative control siRNA or each of the TF-specific siRNAs was carried out to evaluate TF knockdown efficiency at the protein level. Control: protein extracts from THP-1 cells transfected with negative control siRNA. siRNA: protein extracts from THP-1 cells transfected with each TF-specific siRNA. The levels of actin and TATA binding protein (TBP) were also examined as internal references (controls 1 and 2, respectively).
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Tomaru et al. Genome Biology 2009 10:R121 doi:10.1186/gb-2009-10-11-r121