Open Access Research

Genome-wide analysis reveals rapid and dynamic changes in miRNA and siRNA sequence and expression during ovule and fiber development in allotetraploid cotton (Gossypium hirsutum L.)

Mingxiong Pang1, Andrew W Woodward1, Vikram Agarwal1, Xueying Guan1, Misook Ha123, Vanitharani Ramachandran4, Xuemei Chen4, Barbara A Triplett5, David M Stelly6 and Z Jeffrey Chen1237*

Author Affiliations

1 Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USA

2 Institute for Cellular and Molecular Biology, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USA

3 Center for Computational Biology and Bioinformatics, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USA

4 Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA

5 USDA-ARS-SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124, USA

6 Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA

7 Section of Integrative Biology, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USA

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Genome Biology 2009, 10:R122  doi:10.1186/gb-2009-10-11-r122

Published: 4 November 2009

Additional files

Additional data file 1:

Normalized small RNA reads (transcripts per quarter million) per megabase of available repetitive sequences.

Format: XLS Size: 13KB Download file

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Additional data file 2:

Cotton miRNAs and their predicted precursors.

Format: XLS Size: 559KB Download file

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Additional data file 3:

Cotton miRNAs and their targets predicted from ESTs.

Format: XLS Size: 152KB Download file

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Additional data file 4:

Differentially expressed miRNAs in cotton.

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Additional data file 5:

Primers for target cleavage assays and oligonucleotide probes for small RNA blot analysis.

Format: XLS Size: 18KB Download file

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Open Data