Figure 4.

Evolution of mitochondrial localization for the branched-chain-amino-acid aminotransferases family. (a) Gene tree generated by PhyML [70] for vertebrates, yeast and outgroup species that speciated before duplication events. Bootstrap values (100 repetitions) are shown on the internal branches. Proteins surrounded by an oval are localized to mitochondria; loss of the mitochondrial localization is marked by a cross. (b) Clustal W [71] alignment of the amino-terminal region of orthologs. The predicted targeting sequences are highlighted in blue. Abbreviations: hs. Homo sapiens; mm, Mus musculus; dm, Drosophila melanogaster; dr, Dano rerio; lm, L. major; sc, S. cerevisiae.