Open Access Research

Plant polycistronic precursors containing non-homologous microRNAs target transcripts encoding functionally related proteins

Francisco Merchan1,2, Adnane Boualem3*, Martin Crespi1* and Florian Frugier1

1 Institut des Sciences du Végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Avenue de la terrasse, 91198 Gif sur Yvette cedex, France

2 Current address: Dto Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González 2, 41012 Sevilla, Spain

3 Unité de recherche en Génomique Végétale (URGV), Institut National de la Recherche Agronomique (INRA), Rue Gaston Crémieux, 91057 Evry cedex, France

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Genome Biology 2009, 10:R136 doi:10.1186/gb-2009-10-12-r136

Published: 1 December 2009

Additional files

Additional data file 1:

Conserved and non-conserved miRNAs in Arabidopsis, rice and poplar genomes.

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Additional data file 2:

Clustered miRNAs in Arabidopsis, rice and poplar genomes.

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Additional data file 3:

Figure S1: secondary structures of the rice osa-MIR395a-g and osa-MIR395h-l,y putative polycistronic homologous miRNA clusters. Figure S2: secondary structures of poplar and rice putative polycistronic non-homologous miRNA clusters. Figure S3: secondary structures of four Arabidopsis non-homologous polycistronic miRNA clusters: Ath-MIR397b-857, ath-MIR842-846, ath-MIR850-863, and ath-MIR851-771. Figure S4: small RNA hits in Arabidopsis polycistronic non-homologous miRNA clusters based on the 'Genome View' browser in the ASRP database.

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Additional data file 4:

Targets predicted for Arabidopsis non-homologous polycistronic miRNA clusters.

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Additional data file 5:

Primers used in this study.

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