Research
Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression
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* Corresponding author: Juan Larraín jlarrain@bio.puc.cl
1 Center for Cell Regulation and Pathology and Center for Aging and Regeneration, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, 8331150, Chile
2 Laboratorio de Bioinformática Molecular, Depto. Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, 8331150, Chile
Genome Biology 2009, 10:R15 doi:10.1186/gb-2009-10-2-r15
Published: 11 February 2009Additional files
Additional data file 1:
Supplementary Table 1 lists the tags derived from known genes with differential expression along the dorso-ventral axis and their frequency of occurrence in our SAGE libraries. Supplementary Table 2 lists the tag-mapping to transcripts for the 125 tags with significant difference of frequency of occurrence between both libraries. Supplementary Table 3 lists reverse SAGE sequences. Supplementary Table 4 lists the sequences of the primers and information about the probes used in this study.
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Additional data file 2:
The 180 tags with p-values < 0.05 (obtained with the three statistical tests), information about known genes and count fold-ratios.
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Additional data file 3:
RT-PCR for DV11 and DV17 in dorsal and ventral explants of X. tropicalis.
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Additional data file 4:
Experimental tags with their frequencies of occurrence in the SAGE libraries, normalized count ratio and p-values from three different statistical tests.
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Additional data file 5:
The 15-nucleotide tag sequences, their frequencies in the SAGE libraries, count ratios, p-values and their corresponding sequences of 14-nucleotide tags.
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Additional data file 6:
Experimental tags matching to the genome and to the Ensembl transcripts in positions 1, 2 or polyA-next.
Format: TXT Size: 88KB Download file
Additional data file 7:
Experimental tags matching to the genome, and to the NCBI, Unigenes and Gurdon transcript databases in positions 1, 2 or polyA-next (only those not present in Ensembl).
Format: TXT Size: 112KB Download file
Additional data file 8:
Experimental tags matching to the genome but without reliable matches to the transcript databases.
Format: TXT Size: 78KB Download file
Additional data file 9:
Experimental tags matching to the genome but without any match to the transcript databases.
Format: TXT Size: 88KB Download file
Additional data file 10:
Experimental tags matching to the genome and their frequencies of occurrence in the transcript databases used in this study.
Format: TXT Size: 613KB Download file
Additional data file 11:
Tag-mapping of experimental tags to the Ensembl database.
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Additional data file 12:
Tag-mapping of experimental tags to the NCBI database.
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Additional data file 13:
Tag-mapping of experimental tags to the Unigene database.
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Additional data file 14:
Tag-mapping of experimental tags to the Gurdon database.
Format: XLS Size: 2.6MB Download file
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