Open Access Research

High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research

Kathrin Reichwald1*, Chris Lauber14, Indrajit Nanda23, Jeanette Kirschner1, Nils Hartmann1, Susanne Schories2, Ulrike Gausmann1, Stefan Taudien1, Markus B Schilhabel15, Karol Szafranski1, Gernot Glöckner1, Michael Schmid3, Alessandro Cellerino1, Manfred Schartl2, Christoph Englert1 and Matthias Platzer1

Author Affiliations

1 Leibniz Institute for Age Research - Fritz Lipmann Institute, Beutenbergstr., 07745 Jena, Germany

2 Department of Physiological Chemistry I, University of Würzburg, Biozentrum, Am Hubland, 97074 Würzburg, Germany

3 Department of Human Genetics, University of Würzburg, Biozentrum, Am Hubland, 97074 Würzburg, Germany

4 Current address: Department of Medical Microbiology, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands

5 Current address: Institute of Clinical Molecular Biology, University Hospital Schleswig-Holstein, Campus Kiel, Schittenhelmstr., 24105 Kiel, Germany

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Genome Biology 2009, 10:R16  doi:10.1186/gb-2009-10-2-r16

Published: 11 February 2009

Additional files

Additional data file 1:

Coding sequences identified in random genomic sequence samples of medaka, stickleback, tetraodon and zebrafish, their genome sizes, and inferred N. furzeri genome size are given.

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Additional data file 2:

N. furzeri GRZ gene fragments identified by BLASTX searches in Swiss-Prot/TrEMBL.

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Additional data file 3:

Flow cytometry measurements to estimate the N. furzeri genome size.

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Additional data file 4:

G+C histograms of N. furzeri strain MZM-0403 and the closely related species N. kunthae.

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Additional data file 5:

Microsatellite frequencies of N. furzeri GRZ.

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Additional data file 6:

The subgroup of N. furzeri GRZ gene fragments lacking homologs in medaka, stickleback, tetraodon, and zebrafish.

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Additional data file 7:

Chromosomal locations and accession numbers of sequences used for analyses of aging related genes.

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Additional data file 8:

Conservation of the N. furzeri GRZ major rRNA cluster in tetraodon and human.

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Additional data file 9:

Genotypes of microsatellites analyzed in N. furzeri strains GRZ and MZM-0403 and the closely related species N. kunthae.

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Additional data file 10:

Analysis of 19 gene-associated markers in N. furzeri strains GRZ and MZM-0403 and N. kunthae.

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Additional data file 11:

Primers used to clone and sequence five aging related genes in N. furzeri GRZ.

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Additional data file 12:

Nucleotide and amino acid sequences used for phylogenetic analyses.

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