Research
Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology
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* Corresponding author: Buzz Baum b.baum@ucl.ac.uk
1 MRC Laboratory of Molecular Cell Biology, UCL, Gower Street, London WC1E 6BT, UK
2 The Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Genome Biology 2009, 10:R26 doi:10.1186/gb-2009-10-3-r26
Published: 5 March 2009Additional files
Additional data file 1:
Primer sequences used to generate the Drosophila kinase RNAi library and to estimate false positive and false negative rates in the screen.
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Additional data file 2:
This file contains details of the false positive and false negative analysis performed in this study and the effect of dsRNA quality on false negatives.
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Additional data file 3:
Kinases found to be cell morphology hits in each of the six different Drosophila cell lines.
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Additional data file 4:
Actin staining shows similar phenotypes with that of BG3-c2 for mnb RNAi in BG2-c2 and BG3-c1 cell lines.
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Additional data file 5:
Silencing of mnb expression by RNAi causes an average 25% reduction of cell numbers in BG3-c1, BG3-c2 and BG2-c2 cell lines, but has no effect in S2 cells, four days after dsRNA treatment.
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Additional data file 6:
Chart of the gene expression levels determined in the microarray analysis for genes showing phenotypes in both S2R+ and BG3-c2 cells compared to those with phenotypes in BG3-c2 or S2R+ cells. There is no strong pattern of gene expression associated with genes with cell type-specific phenotypes.
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Additional data file 7:
Pie chart summarizing the gene expression profiles (present or absent) of genes showing phenotypes in any cell line. The vast majority of genes with expression data available are either present in all cell lines tested, or absent from all. This suggests that cell type specific phenotypes do not arise simply from expression of different subsets of signaling components.
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Additional data file 8:
Primers used for Q-PCR.
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