Method
A computational approach for genome-wide mapping of splicing factor binding sites
1 Department of Biology, the Technion - Israel Institute of Technology, Haifa 32000, Israel
2 Department of Computer Science, Technion - Israel Institute of Technology, Haifa 32000, Israel
Genome Biology 2009, 10:R30 doi:10.1186/gb-2009-10-3-r30
Published: 18 March 2009Abstract
Alternative splicing is regulated by splicing factors that serve as positive or negative effectors, interacting with regulatory elements along exons and introns. Here we present a novel computational method for genome-wide mapping of splicing factor binding sites that considers both the genomic environment and the evolutionary conservation of the regulatory elements. The method was applied to study the regulation of different alternative splicing events, uncovering an interesting network of interactions among splicing factors.
