False positive rates (FPRs) and false negative rates for the three NGS technologies at simulated varying coverage depths. Performances of (a) Roche 454, (b) Illumina GA, and (c) ABI SOLiD at lower coverage depths were simulated by random subsampling of the reads. Error bars represent the standard deviation over the four samples for ten iterations. The thresholds for a 10% and 50% error rate degradation of the minimum false positive rate are indicated by dashed and dotted lines, respectively, and the corresponding coverage depth reported in dashed and dotted boxes, respectively.
Harismendy et al. Genome Biology 2009 10:R32 doi:10.1186/gb-2009-10-3-r32