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Open Access Research

Identification and functional characterization of cis-regulatory elements in the apicomplexan parasite Toxoplasma gondii

Nandita Mullapudi13*, Sandeep J Joseph1 and Jessica C Kissinger12*

Author Affiliations

1 Department of Genetics, University of Georgia, East Green Street, Athens, Georgia, 30602, USA

2 Center for Tropical and Emerging Global Diseases, University of Georgia, DW Brooks Drive, Athens, Georgia, 30602, USA

3 Current address: Department of Pulmonary Medicine, Albert Einstein College of Medicine, Morris Park Ave, Bronx, New York, NY 10461, USA

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Genome Biology 2009, 10:R34  doi:10.1186/gb-2009-10-4-r34

Published: 7 April 2009

Additional files

Additional File 1:

Table 1: oligonucleotide sequences used in Gateway™ cloning and site-directed mutagenesis. Table 2: genome-wide list of genes that contain a candidate regulatory motif in their upstream region. Table 3: comparison of expected and observed number of motifs genome-wide in upstream and coding regions. Table 4: significant GO category enrichments (raw p-value < 0.05) within each motif-containing gene set.

Format: XLS Size: 274KB Download file

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Open Data

Additional File 2:

The dual transfection and luciferase assay experimental set up.

Format: PDF Size: 501KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data