Validation of EGR-1 enrichment by ChIP-real-time PCR analysis. PCR primers were designed to 50 regions in selected clusters and 8 negative regions without enrichment in CpG islands. Data are relative fold enrichments, calculated by determining the apparent immunoprecipitation efficiency and normalized to the level observed at a control region (mean ± standard deviation, n = 2).
Kubosaki et al. Genome Biology 2009 10:R41 doi:10.1186/gb-2009-10-4-r41