Additional data file 1.
(a) siRNA mediated knockdown of EGR-1 mRNA. EGR-1 mRNA were quantified using quantitative RT-PCR. EGR-1 mRNA levels were normalized to GAPDH mRNA and are presented relative to RNA levels in mock cells. RNA levels are representative of four independent experiments. (b) Effect of siRNA on EGR-1 in THP-1 differentiation. Phase contrast and fluorescence images were taken at the same time. Photographs show transfect efficiency indicated by Alexa Fluor 555 (upper) and typical morphological changes in EGR-1 or control siRNA transfected THP-1 cells at 48 hours after PMA stimulation (lower). The white arrows indicate differentiating THP-1 cells. (c) A sample of sonicated DNA. The sonication conditions were optimized to achieve enrichment of fragments between 150 and 600 bp in length.
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Kubosaki et al. Genome Biology 2009 10:R41 doi:10.1186/gb-2009-10-4-r41