Cohesin binding to a subset of convergent sites along fission yeast chromosome arms. (a) Comparison of the binding patterns of the cohesin subunits Rad21-Pk9 in G2-arrested cells, and Rad21-HA3, Psc3-Pk9 and Pds5-Pk9 in exponentially growing cells. Enrichment of DNA fragments in the immunoprecipitate relative to a whole genome DNA sample is shown along a 100 kb region of fission yeast chromosome 2. Each bar represents the average of 11 oligonucleotide probes within adjacent 250 bp windows. The y-axis scale is log2. Dark grey signals represent significant binding as described . Blue bars above and below the midline represent ORFs transcribed from left to right and opposite, respectively. Convergent sites that are bound, or not bound, by cohesin are marked with green and yellow arrowheads, respectively. (b) The location of Rad21-Pk9 peaks (green), assigned as described in Figure S1 in Additional data file 1, along 1.5 Mb of chromosome 2 is compared to the binding sites of the cohesin loader Mis4/Ssl3 (blue; compare Figure 3) and the distribution of convergent sites (Conv., purple).
Schmidt et al. Genome Biology 2009 10:R52 doi:10.1186/gb-2009-10-5-r52