Phylogenetic classification of Escherichia coli O157:H7 strains of human and bovine origin using a novel set of nucleotide polymorphisms
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* Corresponding author: James L Bono jim.bono@ars.usda.gov
- Equal contributors
1 United States Department of Agriculture (USDA), Agricultural Research Service (ARS), US Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, NE 68933, USA
2 USDA, ARS, Western Regional Research Center, Buchanan St, Albany, CA 94710, USA
3 Washington State University, Department of Pathology, Bustad Hall, Pullman, WA 99164-7040, USA
4 Current address: University of Nebraska, Great Plains Veterinary Educational Center, Clay Center, NE 68933, USA
Genome Biology 2009, 10:R56 doi:10.1186/gb-2009-10-5-r56
Published: 22 May 2009Additional files
Additional data file 1:
STEC O157 strains used in this study with their corresponding PFGE patterns and polymorphism-derived genotypes.
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Additional data file 2:
Nucleotide polymorphism allele frequencies in STEC O157 strains of bovine and human origin.
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Additional data file 3:
STEC O157 genotypes defined by 178 nucleotide polymorphisms.
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Additional data file 4:
STEC O157 genotypes defined by a minimal set of 32 nucleotide polymorphisms.
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Additional data file 5:
The triplicate sets of numbers on the tree represent bootstrap values from neighbor-joining, parsimony, and maximum-likelihood algorithms, respectively. Asterisks represent bootstrap values below 50%. The outer taxonomic unit genotype numbers correspond with genotype sequences recorded in Additional data file 4. The outer taxonomic units are color coded by genotype for the tir 255 T>A polymorphism and host origin. The scale bar represents substitutions per site.
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