Genome Biology

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Open Access Research

Phylogenetic classification of Escherichia coli O157:H7 strains of human and bovine origin using a novel set of nucleotide polymorphisms

Michael L Clawson1, James E Keen1,4, Timothy PL Smith1, Lisa M Durso1, Tara G McDaneld1, Robert E Mandrell2, Margaret A Davis3 and James L Bono1*

Author Affiliations

1 United States Department of Agriculture (USDA), Agricultural Research Service (ARS), US Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, NE 68933, USA

2 USDA, ARS, Western Regional Research Center, Buchanan St, Albany, CA 94710, USA

3 Washington State University, Department of Pathology, Bustad Hall, Pullman, WA 99164-7040, USA

4 Current address: University of Nebraska, Great Plains Veterinary Educational Center, Clay Center, NE 68933, USA

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Genome Biology 2009, 10:R56 doi:10.1186/gb-2009-10-5-r56

Published: 22 May 2009

Additional files

Additional data file 1:

STEC O157 strains used in this study with their corresponding PFGE patterns and polymorphism-derived genotypes.

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Additional data file 2:

Nucleotide polymorphism allele frequencies in STEC O157 strains of bovine and human origin.

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Additional data file 3:

STEC O157 genotypes defined by 178 nucleotide polymorphisms.

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Additional data file 4:

STEC O157 genotypes defined by a minimal set of 32 nucleotide polymorphisms.

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Additional data file 5:

The triplicate sets of numbers on the tree represent bootstrap values from neighbor-joining, parsimony, and maximum-likelihood algorithms, respectively. Asterisks represent bootstrap values below 50%. The outer taxonomic unit genotype numbers correspond with genotype sequences recorded in Additional data file 4. The outer taxonomic units are color coded by genotype for the tir 255 T>A polymorphism and host origin. The scale bar represents substitutions per site.

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