Rpd3p is required for proper Msn2/4p action. (a-c) Gene expression measured in wild-type (WT), rpd3Δ, msn2Δ /msn4Δ, and msn2Δ /msn4Δ /rpd3Δ cells treated with 0.4 mM H2O2 for 30 minutes. Average log2 expression changes of (a) 215 genes equally affected by deletion of RPD3, MSN2/MSN4, or MSN2/MSN4/RPD3, (b) 83 genes affected more by deletion of MSN2/MSN4 than RPD3, and (c) 103 genes that display additive dependence on RPD3 and MSN2/MSN4. The standard deviation of the genes' expression is shown for each gene group. (d) Msn2p binding before and 10 minutes after 0.4 mM H2O2 treatment in wild-type and rpd3Δ cells, according to the key for: TSA2 (from (a)), DDR2 (from (b)), YGP1 (from (c)), HOR7 (dependent on Rpd3p only), and YPS127W (dependent on Msn2/4p but not Rpd3p). Fold-change in Msn2p occupancy between stressed and unstressed cells is listed below each plot. Error bars represent the standard deviation of triplicate experiments.
Alejandro-Osorio et al. Genome Biology 2009 10:R57 doi:10.1186/gb-2009-10-5-r57