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Closing gaps in the human genome using sequencing by synthesis

Manuel Garber1, Michael C Zody12, Harindra M Arachchi1, Aaron Berlin1, Sante Gnerre1, Lisa M Green1, Niall Lennon1 and Chad Nusbaum1*

Author Affiliations

1 Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA

2 Department of Medical Biochemistry and Microbiology, Uppsala University, SE-751 24 Uppsala, Sweden

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Genome Biology 2009, 10:R60  doi:10.1186/gb-2009-10-6-r60

Published: 2 June 2009

Additional files

Additional data file 1:

Tables S1: gap region information, including location, name, primers used to amplify sequence, original (HGP) size, and size after closing. Tables S2: clones flanking the gaps in HGP NCBI build 36. Figures S1 and S2 show gel electrophoresis images with approximate amplified region sizes.

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Additional data file 2:

New clones that closed type III gaps as reported in Bovee et al. [3] are shown in bold italics.

Format: XLS Size: 36KB Download file

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Open Data