Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Open Access Highly Accessed Method

Closing gaps in the human genome using sequencing by synthesis

Manuel Garber1, Michael C Zody12, Harindra M Arachchi1, Aaron Berlin1, Sante Gnerre1, Lisa M Green1, Niall Lennon1 and Chad Nusbaum1*

Author Affiliations

1 Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA

2 Department of Medical Biochemistry and Microbiology, Uppsala University, SE-751 24 Uppsala, Sweden

For all author emails, please log on.

Genome Biology 2009, 10:R60  doi:10.1186/gb-2009-10-6-r60

Published: 2 June 2009

Additional files

Additional data file 1:

Tables S1: gap region information, including location, name, primers used to amplify sequence, original (HGP) size, and size after closing. Tables S2: clones flanking the gaps in HGP NCBI build 36. Figures S1 and S2 show gel electrophoresis images with approximate amplified region sizes.

Format: DOC Size: 338KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional data file 2:

New clones that closed type III gaps as reported in Bovee et al. [3] are shown in bold italics.

Format: XLS Size: 36KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data