Method
Closing gaps in the human genome using sequencing by synthesis
1 Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
2 Department of Medical Biochemistry and Microbiology, Uppsala University, SE-751 24 Uppsala, Sweden
Genome Biology 2009, 10:R60 doi:10.1186/gb-2009-10-6-r60
Published: 2 June 2009Additional files
Additional data file 1:
Tables S1: gap region information, including location, name, primers used to amplify sequence, original (HGP) size, and size after closing. Tables S2: clones flanking the gaps in HGP NCBI build 36. Figures S1 and S2 show gel electrophoresis images with approximate amplified region sizes.
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Additional data file 2:
New clones that closed type III gaps as reported in Bovee et al. [3] are shown in bold italics.
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