Additional data file 2.

Mean miRNA CV value for (a) the 50% least variable and (b) 50% most variable miRNAs when no normalization is applied, stable small RNA control normalization is applied, mean expression value normalization is applied or normalization with miRNAs/small RNA controls resembling the mean is applied. (a) All three normalization strategies result in a significant decrease of the mean CV value. (b) Only mean expression value normalization and normalization with miRNAs/small RNA controls resembling the mean result in a significant decrease of the mean CV value. Stable small RNA controls for the T-ALL samples: RNU24, RNU44, RNU48, RNU58A, U18 and Z30; for the leukemias with EVI1 overexpression: RNU6B, RNU24 and RNU58A; for the normal bone marrow samples: RNU44, RNU24 and RNU48; and for the normal human tissues: RPL21, RNU38B and RNU24. MiRNAs/small RNA controls that resemble the mean expression value are listed in Table 1.

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Mestdagh et al. Genome Biology 2009 10:R64   doi:10.1186/gb-2009-10-6-r64