Additional data file 8.

Average fold change expression difference of all miRNAs with an expression below the Cq cutoff of 35 PCR cycles in MYCN amplified neuroblastoma samples compared to MYCN single copy neuroblastoma samples. Fold changes were calculated upon stable small RNA control normalization (black) and mean expression value normalization (orange). Plotted fold changes are log2-based and sorted from positive (upregulated in MYCN amplified tumor samples) to negative (downregulated in MYCN amplified tumor samples). Dashed lines represent a two-fold expression difference. Arrows indicate the threshold between up- and downregulated miRNAs for both normalization strategies (the number of up- and downregulated miRNAs is indicated left and right of each arrow, respectively).

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Mestdagh et al. Genome Biology 2009 10:R64   doi:10.1186/gb-2009-10-6-r64