Additional data file 6.

The first tab contains a graphical representation of identified groups of unknown stress response genes. The roman numerals on the left (I-VI) refer to the network graphs to their right, each representing a group of unknown stress response genes. In the network graphs, brown nodes represent sequences only found in E. siliculosus, while green nodes represent genes found in other organisms also. Edges represent the sequence similarity between two genes (e-value of the BLAST output). The cluster each gene was assigned to by the k-means clustering (Figure 5) is given in boldface. Furthermore, the expression profile of each gene in the three stress conditions (hyposaline-, hypersaline-, and oxidative stress) is given on the right. Yellow indicates that a gene was over-expressed in a particular condition, while black indicates no change, and blue indicates that a particular gene was repressed. The other two tabs contain additional information, such as corresponding genome equivalent, amino acid sequence, subcellular localization of each protein, the results of the transmembrane domain search, and the expression profile for both the genes shown in the first tab and the other stress response genes that could not be assigned to the different groups.

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Dittami et al. Genome Biology 2009 10:R66   doi:10.1186/gb-2009-10-6-r66