Research
Insights into female sperm storage from the spermathecal fluid proteome of the honeybee Apis mellifera
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* Corresponding author: Boris Baer bcbaer@cyllene.uwa.edu.au
1 ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Stirling Hwy, Crawley WA 6009, Australia
2 Centre for Evolutionary Biology, School of Animal Biology, The University of Western Australia, Stirling Hwy, Crawley WA 6009, Australia
3 Centre of Excellence for Computational Systems Biology, The University of Western Australia, Stirling Hwy, Crawley WA 6009, Australia
Genome Biology 2009, 10:R67 doi:10.1186/gb-2009-10-6-r67
Published: 18 June 2009Additional files
Additional data file 1:
MS/MS spectra derived from trypsinated peptides of spermathecal fluid proteins were matched using Mascot (Matrix Sciences) against Honey Bee PreRelease 2.0. In each case: 'MOWSE' is the Mascot reported molecular weight search score (>37 is P < 0.05); 'Peptides' is the number of peptides matched to the protein above the threshold as outlined in materials and methods; and 'Gelspot' refers to the protein band number as shown in Figure 1. Shading indicates multiple matching data to the same Apis predicted protein sequence from gel bands. Seminal fluid and sperm data on the same proteins are reproduced from [19]. Bee genome GB number and the corresponding RefSeq protein GI from NCBI are given along with the assembly 4.0 gene ID for the bee genome.
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Additional data file 2:
MS/MS spectra derived from trypsinated peptides of spermathecal fluid proteins were matched using Mascot (Matrix Sciences) against Honey Bee PreRelease 2.0. In each case 'Spectra' is the number of spectra matching to each protein as outlined in Materials and methods. Bee genome GB number and the corresponding RefSeq protein GI from NCBI are given.
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Additional data file 3:
Visualization of spermathecal and seminal fluid metabolic networks based on the proteins identified in this study and Baer et al. [19]. Colored nodes (rounded squares) represent enzymes in different functional categories, metabolites are shown as small grey circles, and reactions are shown as connecting lines between the enzyme and metabolite nodes. EC numbers are listed near enzyme nodes and metabolite names for all features are noted. The seven enzymes in common between the two datasets are highlighted by increased size, red outlines and consistent spatial arrangement in both networks.
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Additional data file 4:
Abundances are fluorescence signals from microarrays of virgin and mated spermatheca compared to the average signal in the whole fly average data. Data are derived from [41].
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