Research
Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea
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* Corresponding author: Fabrice Confalonieri fabrice.confalonieri@u-psud.fr
- † Equal contributors
1 Laboratoire de Génomique des Archae, Université Paris-Sud 11, CNRS, UMR8621, Bât400 F-91405 Orsay, France
2 CEA, DSV, IBEB Laboratoire de Biochimie des Systèmes Perturbés, Bagnols-sur-Cèze, F-30207, France
3 CEA, DSV, Institut de Génomique, Genoscope, rue Gaston Crémieux CP5706, F-91057 Evry Cedex, France
4 Laboratoire de Biologie moléculaire du gène chez les extrêmophiles, Université Paris-Sud 11, CNRS, UMR8621, Bât 409, F-91405 Orsay, France
Genome Biology 2009, 10:R70 doi:10.1186/gb-2009-10-6-r70
Published: 26 June 2009Additional files
Additional data file 1:
All T. gammatolerans genes (Table S1); conserved genes among the three sequenced Thermococcus species (Table S2); conserved genes in the six sequenced Thermococcales (Table S3); the T. gammatolerans genes absent in other Thermococcales (Table S4); and genes present in all sequenced Thermococcus species but absent in all Pyrococcus species (Table S5).
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Additional data file 2:
All the non-redundant peptides (Table S6) and proteins (Table S7) identified in T. gammatolerans; spectral count comparison of proteins detected in exponential and stationary phases per class and fold change (Table S8).
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Additional data file 3:
The 290 amino-terminal peptide signatures identified in T. gammatolerans (Table S9) representing 178 non-redundant amino-terminal events (Table S10).
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Additional data file 4:
Auxotrophic requirement of T. gammatolerans deduced from genome analysis and auxotrophic assays (Table S11).
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Additional data file 5:
Detailed legend to Figure 5, including gene IDs.
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Additional data file 6:
Subunit content of the soluble heterotetrameric hydrogenases Mbh and Mbx in several Thermococcale species (Table S12); T. gammatolerans Mbc1 (tg0048 to tg0054) and Mbc2 (tg1241 to tg1249) complex subunits and their respective homologues (Table S13); formate hydrogenlyase 1 subunit content and their respective homologues found in other Thermococcale species and in E. coli (Table S14); formate hydrogenlyase 2 subunit content and the formate transporter (Foc) (Table S15); and hydrogenase maturation proteins as well as homologous genes found in other sequenced Thermococcale species (Table S16).
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Additional data file 7:
Protein sequence alignments of the putative additional MhyI subunits.
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Additional data file 8:
T. gammatolerans genes conserved in D. radiodurans and Deinococcus geothermalis.
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