Table 3

Carbon sources and S° requirements of T. gammatolerans EJ3

Growth

Carbon source

Media

Without S°

With S°

Yeast extract and tryptone

VSM, MAYT

-

+++

20 amino acids

ASW-AA

-

++

Casamino acids

ASW-CASA

-

-

Yeast extract

ASW-YE

-

+++

Tryptone

ASW-T

-

+++

Peptone

ASW-P

-

+++

Pyruvate

ASW-AA-Pyr

++

++

Pyruvate

MAYT-P

+++

+++

Starch

MAYT-S

+

+++

Maltodextrins

MAYT-Mdx

+

+++

Maltose

MAYT-M

-

+++

Trehalose

MAYT-T

-

+++

Glucose

MAYT-G

-

+++

Lactose

MAYT-L

-

+++

Serum bottles were inoculated at a final concentration of 5 × 10⁵ cells/ml and incubated at 85°C. Growth was recorded during 3 days. All tests were performed in triplicate. Final cellular density reached at the stationary phase: +++, >5 × 10⁸ to 10⁹ cells/ml of culture; ++, 1 to 2 × 10⁸ cells/ml of culture; +, 5 × 10⁷ cells/ml of culture; -, no growth.

Zivanovic et al. Genome Biology 2009 10:R70   doi:10.1186/gb-2009-10-6-r70