Table 4 |
|||||
|
High scoring and corresponding low scoring multi-species conserved elements |
|||||
|
Cluster |
Gene |
MCE |
Score |
Control |
Score |
|
|
|||||
|
1 |
CAMK2N1 |
CAMK2N1.3 |
548.29 |
No control MCE* |
|
|
CAMK2N1.4 |
152.32 |
||||
|
1 |
SCAMP5 |
SCAMP5.1 |
476.64 |
SCAMP5.7 |
0 |
|
SCAMP5.30 |
90.5 |
||||
|
1 |
SNAP25 |
SNAP25.78 |
479.98 |
SNAP25.76 |
0 |
|
1 |
STXBP1 |
STXBP1.7 |
479.61 |
STXBP1.1 |
0 |
|
1 |
RAB3C |
RAB3C.32 |
96.13 |
RAB3C.28-9 |
0 |
|
1 |
SYN1 |
SYN1.11 |
1006.13 |
No control MCE* |
|
|
SYN1.10 |
234.92 |
||||
|
1 |
RAB3A |
RAB3A.MCE1 |
997.88 |
No control MCE* |
|
|
RAB3A.MCE2 |
98.63 |
||||
|
2 |
CAMK2G |
CAMK2G.23 |
520.43 |
CAMK2G.22 |
0 |
|
2 |
NAPG |
NAPG.18 |
522.09 |
NAPG.7 |
0 |
|
3 |
CAMK1G |
CAMK1G.523 |
639.59 |
CAMK1G.528 |
0 |
|
3 |
RIMS1 |
RIMS1.394 |
680.61 |
RIMS1.389 |
0 |
|
3 |
STX18 |
STX18.12* |
627.98 |
STX18.15 |
0 |
|
3 |
SYT8 |
SYT8.4 |
508.56 |
SYT8.2 |
0 |
|
|
|||||
|
The list of 16 high scoring multi-species conserved elements (MCEs) and 10 low scoring MCEs for experimental validation (see Materials and method for selection detail). *Genomic regions upstream of CAMK2N1, SYN1 and Rab3A genes do not contain low-scoring MCEs. One of the high-scoring MCEs (STX18.12) failed to be cloned. A Luciferase assay in the Neuro2a cell line identified neuronal enhancers in 12 high-scoring MCEs and 3 low-scoring MCEs marked in bold (Student's t-test, P < 0.05). |
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|
Liu et al. Genome Biology 2009 10:R72 doi:10.1186/gb-2009-10-7-r72 |
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